![]() One of its most common applications is in the ELISA procedure. TMB (tetramethylbenzidine) is a chromogenic substrate that is common in numerous staining procedures used at the Horseradish Peroxidase (HRP) detection step. Choosing the best substrate for your assay will allow the assay developer to create a robust assay with superior performance.įor more information on our recommendations for ELISA substrates, visit the link below. Each TMB substrate impacts these parameters differently. Key assay parameters, such as dynamic range, kinetic rate and assay timing are important factors to consider when evaluating various TMB substrates. Assay timing can be optimized by choosing a TMB substrate with the kinetic rate that allows your assay to be performed within the desired timeframe. In addition, the timing of your assay is important when considering factors such as dynamic range, reproducibility and detection limit. As such, Surmodics IVD offers several TMB substrates at varying kinetic rates to ensure we have an option suitable for each unique assay in question. Kinetic rate is also an important criterion when finding the right substrate for your ELISA. Several key elements can be important such as dynamic range, kinetic rate, and assay timing.ĭynamic range is especially important when developing an assay that requires quantitation of a large range of analyte concentrations. However, the choice of enzyme/substrate can also have a substantial effect on achieving the above-mentioned parameters and require equal attention for optimal selection.ĭuring assay development it is important to consider your substrate selection carefully and to have an understanding of your assay’s requirements. During the optimization process, antibody/antigen systems are carefully chosen to provide the specificity and sensitivity of the desired analyte measurement. In the following section we will take a deeper look at our line of ELISA Substrates specifically.ĭetection limit, dynamic range, and reproducibility are cornerstones in the development of a successful immunoassay application. Our extensive product portfolio includes protein stabilizers, blockers, diluents, antigens and antibodies, protein stability assay, tmb peroxidase substrate, stop solutions, substrates and more. Learn more about how immunoassay tests work. Surmodics IVD provides assay developers a one-stop-shop for immunoassay development, including ELISA’s. These protein-based assays use a variety of immunoassay components. For decades, this plate-based assay has played a major role in identifying and quantifying disease. ![]() ELISA’s (enzyme-linked immunosorbent assay) have been an incredibly useful method in detecting disease for several years.
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